Development of amperometric biosensor for choline determination
نویسندگان
چکیده
منابع مشابه
AMPEROMETRIC MICROBIOSENSOR FOR THE DETERMINATION OF CHOLINE
The construction and performance of an amperometric microbiosensor for the determination of choline chloride by immobilizing choline oxidase on a cellulose acetate membrane are described. The procedure of choline determination involved the electrochemical detection of enzymatically generated hydrogen peroxide. This microsensor has a linear response between 0.038-0.1 mM choline chloride. The...
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Choline oxidase (ChOx) was chosen as a model enzyme for evaluating the performance of CNTs’ functional groups for development of enzyme electrodes. CNTs were functionalized with carboxylic acid, amine or amide groups. Carboxylic acid, amine and amide functionalized CNTs were obtained by acid treatment, ethylenediamine or tetraethylenepentamine chemically modification and ammonia plasma treatmen...
متن کاملevaluation of different functionalized cnts for development of choline amperometric biosensor
choline oxidase (chox) was chosen as a model enzyme for evaluating the performance of cnts’ functional groups for development of enzyme electrodes. cnts were functionalized with carboxylic acid, amine or amide groups. carboxylic acid, amine and amide functionalized cnts were obtained by acid treatment, ethylenediamine or tetraethylenepentamine chemically modification and ammonia plasma treatmen...
متن کاملamperometric microbiosensor for the determination of choline
the construction and performance of an amperometric microbiosensor for the determination of choline chloride by immobilizing choline oxidase on a cellulose acetate membrane are described. the procedure of choline determination involved the electrochemical detection of enzymatically generated hydrogen peroxide. this microsensor has a linear response between 0.038-0.1 mm choline chloride. the res...
متن کاملComputational modeling of amperometric choline biosensor for neurochemical detection
The behavior of biosensors is based on the well-defined physical, chemical and biological reactions, which are expressed by nonlinear differential equations. The characteristics of diffusion reaction of choline biosensor are analyzed mathematically for wide range of input parameters such as initial concentration of choline and thickness of choline oxide enzyme layer. The mathematical simulation...
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ژورنال
عنوان ژورنال: Biopolymers and Cell
سال: 2016
ISSN: 0233-7657,1993-6842
DOI: 10.7124/bc.000925